2´-O-Methoxyethyl (2´-MOE) Panels
2´-O-Methoxyethyl (2´-MOE) Panels
Advance your oligonucleotide therapeutic development with ModDetect™ 2′-MOE panels. These validated antibodies are designed to detect and visualize 2′-MOE–modified antisense oligonucleotides (ASOs) in cells and tissues independent of sequence, enabling consistent, quantitative evaluation of delivery and distribution across preclinical models.
Optimizing ASO Drugs with 2'-MOE Modifications
2′-O-Methoxyethyl (2′-MOE) chemical modification of oligonucleotides is a second-generation modification used to improve the performance of antisense oligonucleotides (ASOs), small interfering RNA (siRNA), transfer RNA (tRNA), messenger RNA (mRNA), and other nucleic acid modalities. MOE modification increases oligonucleotides stability and reduces toxicity compared to phosphorothioate (PS) modifications alone, and is often combined with other chemical modifications to optimize pharmacokinetic and pharmacodynamic properties.
ModDetect™ 2’-MOE panels feature highly specific antibodies for the MOE bond that detect oligonucleotide drugs independent of nucleic acid sequence. These reagents enable sensitive detection and quantification of MOE-modified oligonucleotides of various modalities (ASOs, siRNA, tRNA, mRNA). Anti-2’-MOE antibodies are validated for determining biodistribution, intracellular localization, and quantification and may also be used in immunoassays such as colocalization studies, trafficking, and compartmentalization studies for evaluating RNA therapeutic delivery and activity.
Validated Applications of 2′-MOE Panels in ASO Characterization
ModDetect™ 2′-MOE panels provide powerful, sequence-independent tools for tracking and characterizing 2′-MOE–modified ASOs in biological systems. From biodistribution to intracellular co-localization, these validated assays deliver high specificity and reproducibility, helping drug developers generate actionable insights into oligonucleotide delivery and performance.
Explore our Poster: Antibody-based Analytical Assays for the Localization, Quantification, and Trafficking of Oligonucleotide Therapeutic Drugs in Serum, Cells and Tissues
Biodistribution of 2'MOE-Modified ASOs
Evaluating tissue distribution of MOE-modified ONTs is critical for assessing delivery efficiency, off-target accumulation, and pharmacological activity. Reliable detection in complex tissues guides preclinical candidate selection and supports translational development.
In mouse liver tissue, Anti-MOE antibody clone MOE4 (green) detected accumulation of MOE-modified ASOs in non-parenchymal cells surrounding hepatocytes. No staining was observed in PBS-treated controls, confirming high specificity. These results demonstrate the reproducibility of ModDetect™ antibodies in tissue biodistribution workflows.
Combined Detection of PS and 2-MOE Modifications
Oligonucleotide therapeutics often incorporate multiple chemical modifications to balance stability, uptake, and activity. ModDetect™ Anti-PS (clone PS03) and Anti-MOE (clone MOE4) antibodies were used together to detect a 2′-MOE-modified ASO 5-10-5 gapmer containing ten PS bonds following subcutaneous delivery in mice (50 mg/kg, 72 h).
Both antibodies produced strong, specific staining patterns consistent with the expected biodistribution of the modified ASO, confirming the compatibility of ModDetect reagents for dual-modified oligonucleotide analysis in vivo.
Co-Localization of MOE-Modified ASOs
Co-localization studies are essential for confirming that MOE-modified ONTs are properly internalized and trafficked. Overlap between therapeutic oligonucleotides and Anti-MOE staining validates antibody specificity and provides mechanistic insights into cellular uptake.
In HeLa cells, Cy5-conjugated PS-MOE ASOs (magenta) were co-detected with Anti-MOE antibody clone MOE-C (red). Strong overlap confirmed faithful recognition of MOE modifications and alignment with therapeutic ASO signals. These results validate the use of ModDetect™ Anti-MOE antibodies for subcellular localization studies.
Available ModDetect™ Panels and Clones
The ModDetect™ 2’-MOE Panels are available in unconjugated and biotinylated formats. Individual clones are also available for separate purchase to support custom assays or specialized workflows.
| 2’-MOE Panels | Format |
| ModDetect™ 2´-O-Methoxyethyl (2´MOE) Panel | Unconjugated |
| ModDetect™ 2´-O-Methoxyethyl (2´MOE) Biotinylated Panel | Biotin |
| MOE Clones | Included in Panel |
| Unconjugated Clone MOE1 | ✓ |
| Unconjugated Clone MOE3 | ✓ |
| Unconjugated Clone MOE4 | ✓ |
| Unconjugated Clone MOE9 | ✓ |
| Unconjugated Clone MOEC | ✓ |
| Biotin Clone MOE1 | ✓ |
| Biotin Clone MOE3 | ✓ |
| Biotin Clone MOE4 | ✓ |
| Biotin Clone MOE9 | ✓ |
| Biotin Clone MOEC | ✓ |