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PARP1 (internal) Antibody
Rabbit Polyclonal
1 References
200-401-X51S
200-401-X51
25 µL
100 µg
Liquid (sterile filtered)
Liquid (sterile filtered)
WB, 2D-PAGE, IP
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-PARP1 (internal) (RABBIT) Antibody - 200-401-X51
rabbit anti-PARP1 Antibody, Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, PPOL
Rabbit
Polyclonal
IgG
Target Details
PARP1 - View All PARP1 Products
Human
Recombinant Protein
PARP1 (internal) purified antibody was prepared from whole rabbit serum produced by repeated immunizations with c-terminus region of human PARP1 autocatalytic domain recombinant protein.
PARP1 (internal) was purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human PARP1 protein. No cross reactivity detected towards other PARP members when using siRNAs against 18 PARP family members. Cross-reactivity with PARP1 from other sources has not been determined.
Application Details
2D-PAGE, WB
IP
- View References
Anti-PARP1 (internal) antibody has been tested by western blotting and nanoimmunoassay (NIA). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 113 kDa in size corresponding to PARP-1 by western blotting in the appropriate cell lysate or extract.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
PARP1 is the primary member of the poly(ADP-ribose) polymerase family, whose function is to signal DNA damage (and to recruit repair proteins) by PARylation. PARP1 is also involved in multiple cell death pathways, including apoptosis, necroptosis, autophagy, and a relatively new pathway termed parthanatos. It has been implicated in a new form of cell death termed parthanatos. PARP1 can also promote tissue survival by shifting the balance of cell death programs between autophagy and necrosis. Clinical studies have shown vulnerability to PARP inhibitors in DNA repair defective cancers. Anti-PARP1 (internal) antibody is useful for researchers interested in cellular processes including DNA damage, transcriptional control, and stem cell identity research.
Sikorski et al. (2018). A high-throughput pipeline for validation of antibodies. Nature Methods
Applications
PAGE-MAP, IP-MS
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
