Human Collagen Type III
COLLAGEN III - Western Blot
SDS-PAGE Results of Human Collagen Type III
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Datasheet

Human Collagen Type III

009-001-105
500 µg
Liquid (sterile filtered)
WB, SDS-PAGE
Human
$475.00 /Per Item
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Product Details

Human Collagen Type III - 009-001-105
Type III collagen, collagen 3, human collagen, Alpha 1 type III collagen, Alpha1 (III) collagen, COL3A1_HUMAN, Collagen Type III Alpha 1 Chain, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, Fetal Collagen, Human placenta Collagen Type III Protein, Human Type III Collagen Protein, scleroprotein collagen
Human
Native Protein

Target Details

COL3A1  - View All COL3A1 Products
Human Collagen Type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.  This product reacts with Anti-Collagen Type III.  Reaction with Rockland’s Anti-Collagen I, II, IV, V or VI is negligible.
P02461 - UniProtKB
NP_000081.1 - NCBI Protein
1281 - Gene ID

Application Details

SDS-PAGE, WB
Human Collagen Type III purified protein standard has been tested in SDS-Page and western blot and is used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation, and for other immunological assays as optimized by the user. Specific conditions should be optimized by user. Collagen Type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.

Formulation

1.0 mg/ml by nanodrop at 205 nm
0.5 M Acetic Acid
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is six (6) months from date of receipt.
In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6% of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Collagen type III, a fibrous scleroprotein found in bone, cartilage, tendon, bone marrow stroma and other connective tissue, yields gelatin on boiling. Collagen Type III scleroprotein is a simple protein found in horny and cartilaginous tissues and in the lens of the eye.

What is the source of the collagen proteins?

Collagen proteins are purified from various sources including bovine or human placenta, skin, nasal tissue, and knee cartilage. The purity of the protein is analyzed by SDS-PAGE.

How are the collagen proteins purified?

The proteins were purified by a proprietary method that includes mechanical separation and salt precipitation. Due to the nature and close association of certain collagen proteins, it is difficult to fully separate each collagen.

In what format are the collagen proteins provided?

Collagen proteins are provided in liquid format at a concentration of around 1 mg/mL. The collagen proteins are solubilized, maintaining their native structure. No enzymatic treatment is performed. The low pH of the buffer is essential in maintaining the solubility of the collagen protein.

In what buffer are the collagen proteins provided?

0.5 M Acetic Acid, pH 2.5 with 0.01% (w/v) Sodium Azide.

What are the recommended SDS-PAGE conditions for collagen proteins?

The recommended buffer for SDS-PAGE is 2X SDS buffer with 8 M Urea. The low pH of the buffer maintains the solubility of the collagen proteins. Also, low-density gels such as 3-8% Tris Acetate should be used. Collagen proteins can be reduced with DTT.

What are the recommended storage conditions for collagen proteins?

Collagen proteins should always be stored at 4°C. It is important to never let the collagen proteins freeze because this will degrade the protein. Please be sure to maintain 4°C temperature during assays such as SDS-PAGE.

How was the concentration determined?

The concentration of the collagen proteins was measured by absorbance via nanodrop at 205 nm. When measuring concentration, please be sure to blank with the same buffer as the protein product (0.5 M Acetic Acid).

Are collagen proteins suitable for cell culture?

Since no testing for endotoxin or other contaminants was performed, we do not recommend the use of this product for cell culture.

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No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.; This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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